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Formoso, G

Real-time PCR RNA was extracted by Trizol according to the manufacturers instruction (Biotech) and was reversely transcribed to cDNA by using Primer Script RT reagent Kit (Thermo)

Finally, establishing a standardized MALDI matrix database, along with shared repositories for matrix-related protocols and spectral data, will significantly enhance data accessibility, improve research consistency, and accelerate the development of quantitative imaging technologies