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Detection of intracellular ROS ROS was measured using the fluorescent probe 2,7-dichlorofluorescin diacetate (DCFH-DA, Sigma), which can be hydrolyzed to DCFH within cells

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As for the mechanisms of hypotensive effect beyond BW, augmentation of natriuresis by inhibiting sodium hydrogen exchanger at proximal tubule, ameliorating angiotensin II activity, and enhancing vasodilation can be postulated

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