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RNA extraction, cDNA conversion, and qPCR RNA from 21NT, BT474, IBR.3, and HMEC was extracted, and mRNA gene expression analysis was performed (primers listed in Table 1) using qPCR as described previously (Al-dulaimi et al 2024)

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TIRe demonstrates significant efficacy in reducing glucose levels and promoting weight loss (Chaplin, 2023)

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