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Oxidative stress in autism: elevated cerebellar 3-nitrotyrosine levels

Following this an equal volume of ethyl acetate/trifluoroacetic acid (TFA, 100/1, v/v) was added and after vigorous mixing (5 min) and centrifugation (13,000 x g, 5 min), the supernatants were discarded, and the lower phases were dried in a centrifugal vacuum concentrator

The overlapping mechanisms create risk of excessive sedation and respiratory depression

When you have enough of both, your cells can produce energy efficiently and stay protected while doing so