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Cisbio, 62CGLPEG) according to the manufacturers protocol, and protein was quantified using a BCA Protein Quantification Kit (Invitrogen, A55865)

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This is also why GHK-Cu appears frequently in longevity-adjacent research alongside other compounds studied for age-related cellular changes, since a quantifiable, age-correlated decline gives researchers a concrete variable to test against rather than a purely theoretical one

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One study showed that while niacin acutely lowered FFA and increased GH, chronic use impaired insulin sensitivity [14]