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When only one ice crystal remained, the PBMCs were transferred to a new tube to be washed 2x with warm RPMI (Sigma, St
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On the other hand, in another study of PSD, a high dose of caffeine impairs memory and leads to an anxiogenic and depressive-like behavior in the hole board and elevated plus maze tests, and social interaction and locomotor activity, respectively, also increased pro-inflammatory cytokines such as TNF-alpha and IL-6 [37]
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