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The 2 mg pen the highest available dose, prescribed for patients who require additional glycaemic control beyond 1 mg

Once B12 levels are normalized, patients will likely begin to experience improvements

In brief, 30 ml of the HEK293 cell suspension culture in a 50 ml centrifuge tube (Violamo, #VIO-50BN) was centrifuged sequentially at 1000 g for 10 min and 3000 g for 10 min at 4 C to remove cell bodies and dead cells, respectively, using MX-307 centrifuge and AR510-04 rotor (TOMY, k-factor: 880)