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Complementary DNA (cDNA) was made by reverse transcription of RNA (2 g) using Moloney murine leukemia virus reverse transcriptase (Promega, Madison, MI, USA) and labeled with Biotin-16-2-deoxy-uridine-5-triphosphate (Roche Diagnostics, Indianapolis, IN, USA)

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The published literature spans animal and in vitro models across gastrointestinal, soft-tissue, neurological, vascular, and bone-research endpoints, but it does not establish human therapeutic use