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Pellets were thawed and resuspended in purification buffer (150 mM NaCl, 2 mM MgCl 2 , 50 mM HEPES-KOH pH 7.5, 5 mM -mercaptoethanol, 10% (v/v) glycerol) supplemented with protease inhibitors and DNase I before lysis by sonication

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En el corion superficial se identific una lesin neoplsica en forma de nidos de clulas grandes de ncleos atpicos, vesiculosos con nuclolos prominentes y citoplasma amplio claro con pigmento negruzco en su citoplasma

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