Figure 7A showed the structure of ATT-I
For the following dissolution methods, please prepare the working solution directly: It is recommended to prepare fresh solutions and use them promptly within a short period of time
1 HNMR (300 MHz DMSO- d 6 , ppm): H 12.63 (s, 1H, hydrazine-N H ), 11.65 (s, 1H, 3-ethoxy-4-hydroxybenzylidene-N=C H ), 9.55 (s, 1H, 3-ethoxy-4-hydroxybenzylidene-O H ), 8.34 (s, 1H, 4-hydroxy-3-methoxy benzohydrazide-O H ), 7.91 (m, 2H, 4-hydroxy-3-methoxy benzohydrazideC 2 H and 3-ethoxy-4-hydroxybenzylidene-C 2 H ), 7.31 (brs, 1H, 4-hydroxy-3-methoxy benzohydrazideC 6 H ), 7.11 (brs, 1H, 3-ethoxy-4-hydroxybenzylidene-C 6 H ), 6.87 (s, 1H, 3-ethoxy-4-hydroxybenzylidene-C 5 H ), 6.51 (brs, 1H, 3-ethoxy-4-hydroxybenzylidene-C 5 H ), 4.07 (q, J = 6 Hz, 2H, 3-ethoxy-4-hydroxybenzylidene-OC H 2 CH 3 ), 3.80 (s, 3H, 4-hydroxy-3-methoxy benzohydrazide-OC H 3 ), 1.37 (t, J = 6.0 Hz, 3H, 3-ethoxy-4-hydroxybenzylidene-OCH 2 C H 3 )
Blood Alcohol Concentration (BAC) is the scientific measure to determine the percentage of alcohol present in a persons bloodstream
The homogenates were then centrifuged at 15,000 g for 20 min at 4 C to eliminate remaining waste