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In cognitive peptides: substitute when possible in vivo tests by cerebral organoids or primary neuronal cultures ( Replacement ), optimise experimental plan to minimise the number of animals ( Reduction ), use appropriate anaesthesia and analgesia during invasive procedures ( Refinement )

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1115 In the preconception period, for example, adequate nutrition and levels of micronutrients such as folate, vitamin D, and trace elements, including iron, zinc, and copper, are required for cell division, cellular membrane stability, DNA synthesis, antioxidant capability, and oocyte quality and maturation, all of which are linked to fertility and the potential for implantation