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Next, the protein was eluted using LDOX elution buffer (20 mM potassium phosphate, 200 mM NaCl, 2.5 mM d -desthiobiotin, pH 7.4) and the fractions were combined according to the recorded absorption 280 nm ( A 280nm )

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Astrocytes expressing ALS-linked mutated SOD1 release factors selectively toxic to motor neurons