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To model biotinylated cysteine, a residue was initially parameterized with the General Amber Force Field (GAFF) via OpenMMs residue template generator, then modified to incorporate partial charges from the disulfide-bonded cysteine CYX residue and protein parameters from Amber ff14SB 44

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What the research shows: Chronic ipamorelin treatment increases the volume density of secretion granules in somatotroph cells and modulates intracellular GH content, demonstrating that sustained dosing produces cumulative pituitary-level changes rather than just transient GH spikes (Jimenez-Reina et al., 2002)

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This approach may offer several benefits for long-term research