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Briefly, the Plugin segments individual nuclei, filters them by size, excludes nuclei touching the edges of the field of view, segments single cells using background subtraction and seeded watershed segmentation, and detects lysosomes using the Laplacian of Gauss (LoG) filter and the Find Maxima command

When you are getting people back down to an ideal weight goal rapidly, youre also shortening the amount of time they need to spend on the drug