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Subsequently, the cells were stained with 4 L Annexin V-FITC and 2 L propidium iodide using the Annexin V-FITC Apoptosis Detection Kit (Abcam, Cambridge, UK) and incubated for 15 min at 37 C in the dark

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Wild-type offspring of heterozygous prolactin receptor-null female mice have maladaptive -cell responses during pregnancy

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