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Non-ferroptosis cell death reagents Staurosporine (STS) was purchased from ThermoFisher Scientific (328530010)

In the serum, Fe 2+ is oxidized by ceruloplasmin to Fe 3+ and then binds with transferrin (TF) to form a TFFe 3+ complex, which is endocytosed through the cell membrane transferrin receptor 1 (TFR1) ( 3+ is reduced to Fe 2+ , and the latter is transported out by the divalent metal ion transporter 1 (DMT1) or the zinc-iron-regulatory protein family 8/14 (ZIP8/14) ( 2+ is involved in the unstable iron pool (LIP)

doi: 10.1016/S0140-6736(20)30893-X 31 KuoC-FGraingeMJValdesAMSeeL-CLuoS-FYuK-Het al

Most people use it within 3-4 weeks after mixing

A vitamin B12 shot is an intramuscular injection of cobalamin given directly into a muscle, usually the upper arm or glute