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- However, individuals should monitor their blood sugar levels closely, especially if experiencing GI side effects like vomiting or poor appetite, which may impact glucose stability

Following a washing step, Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:400, Molecular Probes, Waltham, MA, USA) and/or Alexa Fluor 488-conjugated goat anti-mouse IgG (1:400, Molecular Probes) were/was added as the secondary antibody and DAPI was used for nuclear staining

A recent development suggests the use of yeast, mammalian, and insect cell lines over traditional expression systems such as bacteria and fungi for acquiring eukaryotic proteins that require post-translational modifications [2]