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Subsequently, FAD-reconstituted cFSP1, hFSP1, or 6-hydroxy-FAD-reconstituted hFSP1 was eluted from the resin with buffer A supplemented with 250 mM imidazole, dialyzed against a buffer containing 20 mm Tris pH 8.0 and 300 mM KCl, and then frozen at - 80 C for further study