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Afterwards, the supernatants were collected and the fragments snapped frozen prior to homogenization and protein quantification with the Pierce BCA Protein Assay Kit (Prod #23225, ThermoFisher Scientific, France)

1ah (vehicle (isotonic saline), n = 10 mice, 100 nmol kg 1 MK-801 n = 10 mice, 100 nmol kg 1 GLP-1 n = 10 mice, 100 nmol kg 1 GLP-1MK-801 n = 10 mice)

The use of melanotan 2 shots is not approved by the FDA for skin tanning

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