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The resin was washed by ten volumes of wash buffer (50 mM Tris-HCl, pH 8.0, 400 mM NaCl, 1 mM CaCl 2 , 5% glycerol, 0.05% DDM) and mIGF1R protein was eluted by HRV-3C protease cleavage

This can be risky: Although compounded drugs (which are specially mixed medications) are safe in certain circumstances, they may carry safety and efficacy concerns in others

2023;136(12):e233e234

All procedures were in accordance with the ethical standards of the institutional research committee and with the 1975 Declaration of Helsinki and its later amendments

[Google Scholar] 69.Stupnisek M., Kokot A., Drmic D., Hrelec Patrlj M., Zenko Sever A., Kolenc D., Radic B., Suran J., Bojic D., Vcev A., et al