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Zhang et al., 2023), and one used Bloodletting Puncture to treat the sequelae of TBI (Liu et al., 2021)

Metabolic profiling showed that amino acids were highly responsive to 5-oxp and most were up-regulated only under heat stress (serine, tryptophan, alanine, histidine, methionine, aspartate, asparagine, threonine, proline, and glutamine)

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After incubation on ice for 1 h, the homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays