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The resultant pellet was washed and resuspended in ookinete medium (IMDM medium, 50 mg/L of hypoxanthine, and 20% heat-inactivated AB human serum, pH 8.0) to 20% final hematocrit

10.1080/1061186X.2021.1999960 6 ChoiK.LeeY

Three layer gradients of 13%, 16 and 18% (w/v) iodixanol (OptiPrep diluted with HBSS, supplemented with 0.2% BSA (10 ml, 10 ml and 5 ml respectively) are overlaid with the non-parenchymal cells (see Application Sheet C24) in 11% iodixanol and centrifuged at 6,500 g for 30 min [54]

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