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The sections were suffered to antigen retrieval under high temperature and pressure conditions, following which they were treated with the endogenous peroxidase blockers and then blocked in serum for an hour

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In this assay, the 30 min incubation duration was chosen to be optimal because of the inherently cytotoxic nature of CuCl 2 and Cu(OAc) 2 towards HaCaT keratinocytes, as they were observed to cause substantial cell death at a duration longer than 30 mins

Materials and methods 4.1

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