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A comprehensive review published in Biomolecules confirmed that GHK-Cu influences the expression of more than 4,000 human genes, many of them involved in tissue remodeling, antioxidant defense, and anti-inflammatory pathways

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Additionally, various molecular techniques, including Western blotting, reverse transcription, quantitative PCR, immunofluorescence, and immunohistochemistry, were utilized to examine the expression levels of angiogenesis-related factors and the Notch/NF-B pathways-related factors

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The role of ER stress in lipid metabolism and lipotoxicity