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Bands were detected by Novex ECL Chemiluminescent Substrate Reagent Kit (Invitrogen, WP20005) using an ImageQuant LAS 4000 mini (GE Healthcare)

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In India, 5090% of the populationsuffers from vitamin D deficiency, a major health concern

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Note: the specific three-component combination in this blend has not itself been the subject of published clinical or preclinical trials it is offered as a research convenience product, and any additive or synergistic pathway interactions between the three components should be treated as an open research question rather than an established finding