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the lysate was clarified by centrifugation at 15,000 nx g for 15 min, protein concentrations were determined using the BCA total protein assay (Pierce), and then appropriate volumes of the lysates were diluted with 2x reducing Laemmli buffer and heated for ten min at 95 C

The 2011 study mentions that phytoene and phytofluene mediate chronic disease

Dietary carotenoids, also known as -carotene, -carotene, -cryptoxanthin, lutein, zeaxanthin, and lycopene, are the most commonly found carotenoids and are obtained by humans from fruits and vegetables [145]