As with any supplement, it's important to view these tools as additions to a strong foundation, not replacements for it
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Tissues were dissected, post-fixed for 3 h in 4% PFA at room temperature 1820 2 C cryoprotected in 30% (w/v) sucrose in Tris-Azide buffer (40 mM Tris, 10 mM phosphate, 0.7% (w/v) NaCl, 0.05% (w/v) NaN 3 ) at 4 C, then embedded in optimal cutting temperature (OCT) compound, rapidly frozen in isopentane on dry ice, and stored at 80 C until use
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Glutathione is readily transported across cell membranes, so that increasing circulating glutathione levels in blood would facilitate the intracellular uptake of glutathione and alleviate depleted glutathione levels observed in a variety of disease conditions