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Cells were seeded at a density of 10000 cells per well into a 96-well plate in the presence of vehicle, LPS (1 g/mL), LPS (1 g/mL)+exendin-4 (50 nM), or LPS (1 g/mL)+exendin-4 (100 nM) for 10 min (n=3/group)

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Blood was collected from the tail vein of the mice before and 15, 30, 60, 90, and 120 min after injection of glucose, and the whole blood glucose concentration was measured using a Roche glucometer