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First, while the rescue experiments with ferric citrate and NAC strongly support an iron deficiency and ROS-mediated mechanism, the absence of established positive controls, such as the iron chelator deferoxamine (DFO) or direct ROS generators, precludes a definitive comparative conclusion that the observed effects are uniquely attributable to CGAs iron-chelating activity

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The cut-off points for these tertiles were meticulously determined based on the distributions among the control subjects