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It takes at least 20 weeks to reach the maximum 15 mg maintenance dose

The complex was expressed and purified as described above for CDK11-cyclin L-SAP30BP, except that a nickel affinity purification step using Ni-NTA Superflow beads (Qiagen) was added before incubation with the Strep-Tactin Superflow resin, and the cleaved tags and TEV protease were removed by incubation with Ni-NTA Superflow beads before gel filtration

Therefore, the mechanism by which TMAO promotes the development of OA may involve promoting ferroptosis of chondrocytes