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use a titration series with appropriate vehicle and pathway controls
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Reduction of Abdominal and Visceral Fat : Animal and early human models have demonstrated a preferential reduction of abdominal fat following subcutaneous administration of AOD-9604
Reported applications include NNMT enzyme inhibition assays measuring 1-methylnicotinamide formation or S-adenosylhomocysteine release, intracellular target-engagement assays in differentiated 3T3-L1 adipocytes where 5-Amino-1MQ reduced intracellular 1-methylnicotinamide with an EC near 2.3 M (Neelakantan et al., Biochem Pharmacol, 2018), membrane permeability and Caco-2 transport characterisation, methyltransferase selectivity panels covering COMT, DNMT1 and PRMT3, and rodent studies of NNMT inhibition in adipose tissue and liver following the antisense knockdown work of Kraus and colleagues (Nature, 2014)
The research also highlighted a significant reduction in deep abdominal visceral fatthe type of stubborn fat linked to metabolic issues that often resists traditional diet and exercise
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