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Confocal laser scanning microscopy (CLSM) analysis Seeds of two transgenic lines expressing redox-sensitive GFP2 (roGFP2) either in the cytosol (Grx1-roGFP2) or the plastids (TKTP-Grx1-roGFP2) 92 and Col-0 WT seedlings were sown onto 6-well cell culture plates (Sigma-Aldrich, CLS3516) containing 3 ml of liquid MS medium (pH 5.7) supplemented with 2% sucrose

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Results and Discussion Engineering and Validation of the miniGrx1-roGFP2 Biosensor In our hands, the original Grx1-roGFP2 biosensor displayed somewhat inconsistent performance when fused to various targeting sequences for subcellular localization