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Mass spectrometry characterization Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was utilized for the accurate measurement of the molecular weight of mutant nanobodies following the incorporation of ncAAs

In this context, several sensors and pathways regulating the transcription factors, such as activator protein 1 (AP-1), cAMP response element-binding protein (CREB), hypoxia inducible factor-1 (HIF-1), nuclear factor B (NF-B), activating transcription factor 4 (ATF-4), and nuclear factor-erythroid 2-related factor-2 (Nrf2), have been described (Marinho et al

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