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According to the 2018 Autism and Developmental Disabilities Monitoring (ADDM) Network study, the age at first diagnosis ranged from 46 to 67 months [163]
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It is defined as an abnormality in structure or function for at least three months and is a big driver of cardiovascular disease
Samples were then centrifuged at 200 g at room temperature for 5 min, the supernatant was discarded, and the cells were resuspended in PBS
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