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Ko et al found that 8 weeks of treadmill exercise beginning 2 weeks after TBI suppressed the expression of the pro-apoptotic protein Bax and increased the expression of the anti-apoptotic protein Bcl-2 in the hippocampal dentate gyrus of rats and improved spatial learning ability, indicating that treadmill exercise after TBI can inhibit neuronal apoptosis in the brain by suppressing TBI-induced activation of the CREB/BDNF/TrkB signaling pathway after TBI [132]
They demonstrated the binding specificity between SAH-ubiquitin-conjugating enzyme E2 A (SAH-UBE2A) and E1 through a fluorescence microscopy-based qualitative binding assay and examined the specificity for disrupting the transfer of UBE1 thioester in the reconstituted system
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