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Second, although patients with infertility diagnoses were identified, detailed information on fertility treatmentsincluding ovulation induction, intrauterine insemination, in vitro fertilization protocols, cycle characteristics, embryo quality, and hormonal profileswas unavailable

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Plant genomic DNA that had been extracted was measured with a NanoDrop 1000 spectrophotometer (Thermo Scientific) followed by dilution to a 50ng/l concentration

The finding of Ileibacterium as the most abundant cecal microbiome genus in every experimental group is relatively novel and may reflect experimental factors such as diet, housing conditions, and mouse strain