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After completion of the reaction, the pH was adjusted to pH = 5-6 with hydrochloric acid solution (pH = 2)

PRP has (again) gained popularity among a limited number of dermatologists with a primary commercial interest, though the use and clinical validation of the method for diverse dermatologic conditions is still in the early stages

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Those exposed to environmental stressors affecting skin health

A significant change in pH affects the structure of all macromolecules, for instance, presence of a higher proportion of H + or low pH solution cleaves DNA double helix strands by protonating the DNA phosphodiester groups and consequently destabilizing the hydrogen bonds linking DNA nitrogenous bases guanine and cytosine (G-C) (Sorokin et al., 1986)