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Briefly, flash-frozen tissue samples were homogenized in ice-cold PBS containing 2 mM EDTA (1 ml PBS/EDTA per 10 mg tissue) using a Dounce homogenizer

Interestingly, our natural levels of GHK-Cu decline significantly with age, which parallels the loss of skin firmness, slower healing, and thinning hair that so many people notice over time

Figure 1 2 Pathogenesis of RA RA is an inflammatory disease caused by multi-cells communication and interaction by cytokines, which result in a variety of pathological reactions (6)

Fifty microliters of stop solution was added to each well and the optical density at 450 nm determined using a microtiter plate reader (Liuyi, Beijing, China) within 15 min

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