Excess media was removed and replaced with 70% L15 media containing 10 M DCFH-DA and incubated at 20C for 30 min (32), after which the solution was removed, replaced with 10 g/ml Calcofluor White (CFW) to stain Bd for 5 mins (28), rinsed and replaced with 70% L15 media
Talking with an expert who really understands what youre experiencing
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Most GLP-1 side effects are mild and improve as your body adjusts
Tracking your weight and measurements over a couple of weeks, reviewing your eating, sleep, and activity habits, and checking in with your healthcare provider can all help identify whats contributing to the slowdown