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Subsequent biochemical and cytochemical studies demonstrated that HGF nitration is maximal in a physiological pH range at Y198 and Y250, characterized by their respective locations at the c-met binding sites of the K1 and K2 domains of the HGF -chain

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Meanwhile, tissue samples from the duodenum, jejunum, ileum, cecum, and colon were collected, These segments were rinsed with sterile saline and fixed in 4% paraformaldehyde for histological analysis