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In summary, cells were treated with the specified drugs for 24 h, collected, resuspended in PBS, and incubated with 20 M Monochlorobimane for 2 h at 25 C in the dark

The third step in the cycle is the reduction of GSSSSG to regenerate GSSH

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10.1016/j.freeradbiomed.2010.01.028 [DOI] [PMC free article] [PubMed] [Google Scholar] Zielonka J, Hardy M, Kalyanaraman B (2009) HPLC study of oxidation products of hydroethidine in chemical and biological systems: ramifications in superoxide measurements

Thannickal VJ, Fanburg BL2000Reactive oxygen species in cell signaling.1005L1028