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Recombinant 6His-tagged proteins were purified at 4 C from corresponding soluble fractions by affinity chromatography with Ni-NTA agarose resin (GE Healthcare)
[DOI] [PMC free article] [PubMed] [Google Scholar] 282.De Angelis C, Scarf C, Falcinelli M, Perna E, Ramacci MT, Angelucci L
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Ageing of the brain is paralleled by an increase of the glutamate to glutamine ratio indicating abnormal operation of the glutamate (GABA)-glutamine shuttle
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