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Regular sterile water: No preservative Bacteria can grow after opening Reconstituted peptides only safe for 24 hours Must be used same-day Some peptides (like growth hormone) come with sterile water because you're supposed to use the entire vial immediately

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Diabetes, Obesity and Metabolism (2022) 7

Protein Science 17, 240-250

Pain during injection may indicate that the needle has hit a nerve or that the medication is being injected too quickly into shallow tissue