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Several distinct populations of microglia and macrophages have been described in the aging brain, including dark microglia (Kaya et al., 2022), and senescent-like microglia (Gross et al., 2025), underlying the heterogeneity of immune cell responses to aging-related stress

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In practice, gelling usually traces back to three avoidable errors: Diluent that is too cold: Cold liquid can create an abrupt hydration shock

And again, technique also comes into play

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