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IL-23 is indispensable in the survival, amplification and pathogenicity of Th17 cells in the late stage of Th17 differentiation[22].On the contrary, induced Treg (iTreg) cells is driven by high concentrations of TGF-, T-cell receptor (TCR) signaling and IL-2

Del Buono et al., Tendinopathy and inflammation: some truths, International journal of immunopathology and pharmacology 24, 1 Suppl 2 (2011): 45

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The cells were plated on collagen-coated 96-well plates at the density of 2 10 4 per well in 0.1 mL DMEM, treated subsequently as described above