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First, our enteric nervous system (ENS), which is located throughout our gastrointestinal tract, helps control our same chemicals and cells as the brain to help us digest and to alert the brain when something is amiss (Ruder, 2017)

Cefalu WT, Stenlf K, Leiter LA, et al

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The resulting culture was then incubated at 37 C until the OD reached 0.6, after which the temperature was dropped to 18 C and the culture was induced by adding IPTG to a final concentration of 1 mM

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