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Next, the protein was eluted using LDOX elution buffer (20 mM potassium phosphate, 200 mM NaCl, 2.5 mM d -desthiobiotin, pH 7.4) and the fractions were combined according to the recorded absorption 280 nm ( A 280nm )

Abbreviations ME: -mercaptoethanol BrdU: 5-bromo-2-deoxyuridine BSA: Bovine serum albumin DAB: 3,3-diaminobenzidine DMEM: Dulbeccos modified Eagles medium ECL: Enhanced chemiluminescence GSSSG: Glutathione trisulfide HGF: Hepatocyte growth factor HRP: Horseradish peroxidase HS: Horse serum mAb: Monoclonal antibody LASSS: Lipoic acid trisulfide SDS: Sodium dodecyl sulfate TTBS: Polyethylene sorbitan monolaurate (Tween20)-Tris-buffered saline Y: Tyrosine residue References Honda, S

C.EzakiY.MatsumotoH

S2CID 25988269

Collagen Support and Anti-Ageing Oxidative stress is a primary driver of collagen degradation