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Step 9: Visually Inspect the Solution Hold the vial up to a light source and examine the solution before proceeding: Step 10: Aliquot and Label If you will not use the entire reconstituted volume within one experiment session, aliquot into single-use volumes before storage

A small fraction of the cDNA was allocated to real-time PCR quantification using a LightCycler 2.0 (Roche)

Hepatic first-pass elimination (1 Fractional bioavailability) of Drug A is 60 % while that of Drug B is 40 %

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We believe that the inclusion of this statement should help allay commenters' concerns