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This shows that natural homocysteine levels are a U-shaped curve in regard to all-cause mortality, but if there is an external reason for low homocysteine, such as excess NAC or B6, there is no statistically significant negative effect.[ref] Which brings us to the role of the gut microbiome in both low or high homocysteine

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The math works the same way, just with different numbers

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Some LC-MS methods have been reported, where NEM has been selected as alkylating agent, however, again some discrepancies regarding the use of NEM are evident