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Antioxidants (Basel, Switzerland), 9(7)

Property Detail Full Name Thymosin Beta-4 active fragment (TB-500) Amino Acids 43 (full T4 sequence) Active Region LKKTETQ (residues 17-23) Molecular Weight ~4,963 Da (full T4) Origin Thymus gland protein, ubiquitous in nucleated cells CAS Number 77591-33-4 (Thymosin Beta-4) FDA Status Not approved WADA Status Prohibited (S2 category) Is TB-500 FDA Approved

We extracted the mRNA from the aorta of mice in different groups and performed RT-qPCR analysis

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with 100 g TNP-Ficoll FITC (Biosearch Technologies F-1300F) and 30 min later tissues were analyzed with flow cytometry (splenocytes were stained with surface markers for the detection of FoB and MZB, with the addition of biotinylated anti-TNP (BD Pharmingen 554055) + Streptavidin-APC (Biolegend 405207 for TNP detection) or snap-frozen for immunofluorescence microscopy in OCT (Sakura 4583)